Skeletal Phenotyping of Irf8 Knockout Mice

ROSSA-20260926-E232

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Study Information

Completion Date:

January 3, 2018

Publication Information:

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Summary

This screen examines randomly selected homozygous knockout mice generated by the IMPC/KOMP Consortium. The mice were bred and tissues collected at the Bar Harbor Jackson Laboratory facility and transferred to the University of Connecticut Health Center in Farmington, CT where the µCT and bone cryo-histomorphometry evaluation was performed. Batches of C57/BL6 control mice were bred, collected at the Farmington campus and analyzed on a bimonthly basis to match the time that the KOMP were bred and analyzed.

Funding Sources

5R01AR063702

Conflicts of Interest

None

Investigators

Investigator Department Location Role
David Rowe Department of Regenerative Medicine and Skeletal Development University of Connecticut Health CT, United States Corresponding
Seung-Hyun Hong Department of Computer Science University of Connecticut CT, United States Contributing
Caibin Zhang Department of Regenerative Medicine and Skeletal Development University of Connecticut Health CT, United States Contributing
Dong-Guk Shin Department of Computer Science University of Connecticut CT, United States Contributing
Douglas Adams Department of Orthopedics University of Colorado CO, United States Contributing
Peter Maye Department of Regenerative Medicine and Skeletal Development University of Connecticut Health CT, United States Contributing
Cheryl Ackert-Bicknell Department of Orthopedics University of Colorado CO, United States Contributing

Animal Models

Mouse strain name: B6N(Cg)-Irf8<tm1b(KOMP)Wtsi>/J
Genetic Category: Global Knockout/Knockin - Gene Targeting
Knockout (KO)

Strain: C57BL/6NTac

Source of Animal Model: IMPC

Gene: Irf8 - interferon regulatory factor 8

Functional Change: loss_of_function

Mouse C57BL/6NTac
No Genetic Modification

Strain: C57BL/6NTac

Sample Groups

  • Sample Groups /
    • Male /
      • Control Animals /
        • Irf8 WT Male (Group 202)
          • Animal: C57BL/6NTac
          • Genotype: +/+
          • Age: 12wk
      • Experimental Animals /
        • Irf8 KO Male (Group 200)
          • Animal: C57BL/6NTac
          • Genotype: KO/KO
          • Age: 12wk
    • Female /
      • Control Animals /
        • Irf8 WT Female (Group 203)
          • Animal: C57BL/6NTac
          • Genotype: +/+
          • Age: 12wk
      • Experimental Animals /
        • Irf8 KO Female (Group 201)
          • Animal: C57BL/6NTac
          • Genotype: KO/KO
          • Age: 12wk

Comparisons

Comparison Group
Irf8 WT Male Irf8 KO Male
Irf8 WT Female Irf8 KO Female

Phenotype Analysis

Bone Histomorphometry

Bone: Femur, Region: Trabecular

Bone Femur
Region Trabecular
Measurement software Custom
Body weight included Yes
Histological approach Frozen Non-Decalcified Fluorescence
Bone Histomorphometry

Bone: Vertebra, Region: Trabecular

Bone Vertebra
Region Trabecular
Measurement software Custom
Body weight included Yes
Histological approach Frozen Non-Decalcified Fluorescence
Microcomputed Tomography (µCT)

Bone: Femur, Region: Cortical

Bone Femur
Region Cortical
Scan mode Ex Vivo
Voxel size 16 μm
Scanner Scanco μCT40
Body weight included Yes
Optional parameters
X-ray tube potential 55 kVp
Integration time 300 ms
Analysis software —
Microcomputed Tomography (µCT)

Bone: Femur, Region: Trabecular

Bone Femur
Region Trabecular
Scan mode Ex Vivo
Voxel size 16 μm
Scanner Scanco μCT40
Body weight included Yes
Optional parameters
X-ray tube potential 55 kVp
Integration time 300 ms
Analysis software —
Microcomputed Tomography (µCT)

Bone: Vertebra, Region: Trabecular

Bone Vertebra
Region Trabecular
Scan mode Ex Vivo
Voxel size 16 μm
Scanner Scanco μCT40
Body weight included Yes
Optional parameters
X-ray tube potential 55 kVp
Integration time 300 ms
Analysis software —